منابع مشابه
Gene targeting in Physcomitrella patens.
Gene-targeting efficiency in the land plant Physcomitrella patens (Bryophyta) can only be compared with that observed in Saccharomyces cerevisiae. Sequencing programs and microbiological molecular genetic approaches are now being developed to unravel the precise function of plant genes. Physcomitrella patens, as the new 'green yeast', might well become a major tool for functional genomic studie...
متن کاملPhyscomitrella patens
Arabidopsis and other species, this work has also begun to reveal the levels of evolutionary conservation within the flowering plants and even outside the plant kingdom. What does the future hold for Antirrhinum research? In addition to its continuing role as a genetic model for plant biology and development, several new experimental avenues are beginning to be explored with Antirrhinum. A majo...
متن کاملMRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens
The moss Physcomitrella patens is unique among plant models for the high frequency with which targeted transgene insertion occurs via homologous recombination. Transgene integration is believed to utilize existing machinery for the detection and repair of DNA double-strand breaks (DSBs). We undertook targeted knockout of the Physcomitrella genes encoding components of the principal sensor of DN...
متن کاملParameters determining the efficiency of gene targeting in the moss Physcomitrella patens
In the moss Physcomitrella patens, transforming DNA containing homologous sequences integrates predominantly by homologous recombination with its genomic target. A systematic investigation of the parameters that determine gene targeting efficiency shows a direct relationship between homology length and targeting frequency for replacement vectors (a selectable marker flanked by homologous DNA). ...
متن کاملThe mechanism of gene targeting in Physcomitrella patens: homologous recombination, concatenation and multiple integration
The model bryophyte Physcomitrella patens exhibits high frequencies of gene targeting when transformed with DNA constructs containing sequences homologous with genomic loci. 'Targeted gene replacement' (TGR) resulting from homologous recombination (HR) between each end of a targeting construct and the targeted locus occurs when either single or multiple targeting vectors are delivered. In the l...
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ژورنال
عنوان ژورنال: Current Opinion in Plant Biology
سال: 2001
ISSN: 1369-5266
DOI: 10.1016/s1369-5266(00)00150-3